ACT-LABS gun system Instruction Manual download pdf (Page 49)

Fig. 26. Representative cross-section showing penetration of gold particles into water agar after

discharge with a helium pulse.

10.7 Quantitation of DNA in Cartridges

This procedure can be used for estimating the amount of DNA in cartridges when 0.5 µg

or more of DNA has been loaded per tube.

Materials

10 mM Tris, pH 8.0, 1 mM EDTA 1,000 µl micropipettor and tips

Ultrasonic cleaner UV spectrophotometer

Vortexer Quartz cuvettes

Microfuge

Procedure

1. Place five 0.5" cartridges in a microfuge tube and add 500 µl of TE.

2. Sonicate and/or vortex to dislodge gold and solubilize the DNA. Spin for 5 min in a

microfuge to pellet gold.

3. On a spectrophotometer determine the A

260

by reading against a TE blank. If 100% of the

DNA were precipitated onto the microcarriers, the A

260

readings should be as follows:

Plasmid/cartridge A

260

0.5 µg 0.1

1.0 0.2

2.0 0.4

4. DNA sample may also be analyzed by restriction enzyme digestion and analysis by agarose

gel electrophoresis.

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ACT-LABS gun system Instruction Manual Page 49