ACT-LABS gun system Instruction Manual download pdf (Page 43)

Environmental

Operating 50 °F ( 10 °C) to 90 °F (32 °C) temp.

30–80 % humidity

Storage 32 °F (0 °C) to 140 °F (60 °C) temp.

10–90 % humidity

Tubing Prep Station Specifications

Physical

Dimensions Approximately 33.5” x 4”

Weight 11.2 lbs

Construction Aluminum and Acrylic

Electrical

Maximum Current 62 mA/125 mA peak

Voltage Input 220–240 Vac/100–120 Vac

Input Frequency 50/60 Hz

Functional

Input Pressure 30 psi N

Relief Pressure 30 psi ± 1.5 psi at regulator assembly

Regulator Adjustment TDB psi limit maximum

Speed 30 RPM nominal

Tubing Fill manual

Environmental

Operating 50 °F (10 °C) to 90 °F (32 °C) temp

30–80 % humidity

Storage 32 °F (0 °C) to 140 °F (60 °C) temp

10–90 % humidity

Section 10

Appendices

10.1 Precipitation of RNA onto microcarriers

Primary RNA transcripts and mRNA may be effectively delivered in vivo and in vitro

using the Helios Gene Gun System (Qiu et al., 1996). The procedure is similar to that for

DNA, but the precipitation step is performed with ammonium acetate and 2-propanol.

Materials

In addition to those identified in Section 5.2:

Purified RNA preparation (e.g. capped, polyadenylated, or oligo dT-selected mRNA).

5 M ammonium acetate

2-propanol

Experiments involving RNA require careful technique to prevent RNA degradation. In the stud-

ies published to date, it was not necessary to pre-treat the gold particles to prevent RNase contamination.

Procedure (sufficient for one length of Gold-Coat tubing):

1. In a 1.5 ml microfuge tube, weigh out 25 mg gold particles.

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ACT-LABS gun system Instruction Manual Page 43